Literature summary for 2.8.2.1 extracted from

Zhou, Z.; Li, Q.; Huang, H.; Wang, H.; Wang, Y.; Du, G.; Chen, J.; Kang, Z.
A microbial-enzymatic strategy for producing chondroitin sulfate glycosaminoglycans (2018), Biotechnol. Bioeng., 115, 1561-1570 .

Search for more literature for 2.8.2.1

Application

Application	Comment	Organism
synthesis	the enzyme is used for large scale chondroitin sulfate synthesis in a coupled system with chondroitin 4-sulfotransferase and chondroitin 6-sulfotransferase, overview	Rattus norvegicus

Cloned(Commentary)

Cloned (Comment)	Organism
gene Sult1a1, recombinant expression in Bacillus subtilis strain E168C, Escherichia coli strain BL21(DE3), and Pichia pastoris strain GS115, coexpression with C4ST and C6ST from Rattus norvegicus, subcloning in Escherichia coli strain JM109. Chondroitin extraction and quantification	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
additional information	reconstitution of the chondroitin biosynthesis pathway in a recombinant Bacillus subtilis strain using sucrose as carbon source, method optimization, overview. Specific sulfation transformation systems are successfully constructed and optimized by combining the purified aryl sulfotransferase IV (ASST IV), chondroitin 4-sulfotransferase (C4ST, EC 2.8.2.5) and chondroitin 6-sulfotransferase (C6ST, EC 2.8.2.17). Chondroitin sulfate A and C are enzymatically transformed from chondroitin at conversion rates of 98% and 96%, respectively. The ASST IV enzyme is used for PAPS regeneration. Purification of CS disaccharides by anion exchange chrmatography and NMR analysis of the composition of CS disaccharides	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3'-phosphoadenylyl sulfate + phenol	Rattus norvegicus	-	adenosine 3',5'-bisphosphate + phenyl sulfate	-	r

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	P17988	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-phosphoadenylyl sulfate + phenol	-	Rattus norvegicus	adenosine 3',5'-bisphosphate + phenyl sulfate	-	r
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate	-	Rattus norvegicus	3'-phosphoadenylyl sulfate + 4-nitrophenol	-	r

Synonyms

Synonyms	Comment	Organism
aryl sulfotransferase IV	-	Rattus norvegicus
ASSTIV	-	Rattus norvegicus
SULT1A1	-	Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Rattus norvegicus

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Release 2023.1 (January 2023)